Course Meeting Times
Lectures: 2 sessions / week, 1.5 hours / session
Subject Description
This class provides an introduction to the interactions between cells and the surfaces of biomaterials. The course covers: surface chemistry and physics of selected metals, polymers, and ceramics; surface characterization methodology; modification of biomaterials surfaces; quantitative assays of cell behavior in culture; biosensors and microarrays; bulk properties of implants; and acute and chronic response to implanted biomaterials. General topics include biosensors, drug delivery, and tissue engineering.
Prerequisites
8.01; 8.02; 3.091, 5.111, or 5.112; 7.012, 7.013 or 7.014; 3.012, 5.60, or 2.40.
Texts
Ratner, Buddy D., et al. Biomaterials Science: An Introduction to Materials in Medicine. 2nd ed. Burlington, MA: Academic Press, 2004. ISBN: 9780125824637.
Supplemental Handouts.
Homework
Approximately biweekly (7 problem sets).
Tests
Two quizzes and one final exam.
Grading
| ACTIVITIES | PERCENTAGES |
|---|---|
| Homework* | 40% |
| Quiz 1 | 15% |
| Quiz 2 | 15% |
| Final Exam | 30% |
* weighting may vary
Note: Students may discuss homework problems, but should write up solutions independently. Quizzes and final are closed-book exams.
Calendar
Several lectures are given by the course TA Dr. Ikuo Taniguchi.
| SES # | TOPICS | ASSIGNMENTS |
|---|---|---|
| 1 |
Course Introduction Biomaterials Defined Structural Hierarchy in Materials and Biology | Problem set 1 assigned |
| 2 |
Biomaterials Surfaces: Physics Surface (vs. Bulk) Structure and Properties Surface Energy Adsorption, Segregation, and Reconstruction at Surfaces | |
| 3 |
Biomaterials Surfaces: Chemistry Reactions at Surfaces: Chemisorption, Corrosion | |
| 4 |
Biomaterials Surfaces: Chemistry (cont.) Hydrolysis (Dr. Taniguchi) | Problem set 1 due Problem set 2 assigned |
| 5 |
Protein-Surface Interactions Proteins: Structure, Properties, Functions Protein Adsorption: Langmuir Model | |
| 6 |
Protein-Surface Interactions (cont.) Protein Adsorption: Complex Phenomena, Measurement | Problem set 2 due Problem set 3 assigned |
| 7 |
Cell-Surface Interactions: Host Response to Biomaterials Cell Adhesion Mechanisms Coagulation Cascade | |
| 8 |
Cell-Surface Interactions: Host Response to Biomaterials (cont.) Immune Response: Alternative Complement Activation | |
| 9 |
Surface Modification Methods Purposes Plasma Treatments Organic Coatings (Dr. Taniguchi) | Problem set 3 due |
| Quiz 1 | ||
| 10 |
Surface Modification Methods (cont.) Polymer Coatings Patterned Surfaces | Problem set 4 assigned |
| 11 |
Surface Characterization In Vacuum: XPS/ESCA, AES, SIMS | |
| 12 |
Surface Characterization In situ Contact Angle, AFM | Problem set 4 due |
| 13 |
Quantifying Cell Behavior Cell Cultures Cellular Assays: Adhesion, Migration, Proliferation | Problem set 5 assigned |
| 14 |
Quantifying Cell Behavior (cont.) Cellular Assays: Differentiation, Function | |
| 15 |
Statistical Analysis Error Sources, Distribution Functions, t- test | |
| 16 |
Statistical Analysis (cont.) Fisher Test, Regression Methods | Problem set 5 due Problem set 6 assigned |
| 17 |
Biosensors and Diagnostic Devices Biological Elements Transduction Mechanisms Properties Examples: Fiber Optic Biosensors, Nanobarcodes | Problem set 6 due |
| Quiz 2 | ||
| 18 |
Drug Delivery: Controlled Release (Dr. Taniguchi) Chemically-controlled Devices | |
| 19 |
Drug Delivery: Controlled Release (cont.) Diffusion-controlled, Membrane-based and Osmotic Devices | Problem set 7 assigned |
| 20 |
Drug Delivery: Controlled Release (cont.) Methods: Transdermal, Colloidal Vehicles Mechanical Pumps | |
| 21 |
Biomaterials for Organ Replacement Organ Replacement Therapies Mechanical Properties Bone Substitutes | |
| 22 |
Tissue Engineering Cell Types; Approaches: In vitro, In vivo, Ex vivo/Cell Encapsulation Scaffolds: Design and Fabrication | Problem set 7 due |
| 23 |
Tissue Engineering (cont.) Case Examples: Artificial Pancreas, Cartilage, Nerve Regeneration | |
| 24 | FDA Regulatory Issues and Course Evaluations | |
| Final Exam |